mirna_target = ["UACAGUACUGUGAUAACUGAA"] #polyAdenaltion function def polya ( mirna ): return mirna + 'A' * 30 mirna_target_a = polya(mirna_target[0]) print "Micro RNA Target: " + mirna_target[0] print "Poly A: " + polya( mirna_target[0]) #RNA to cDNA mirna_target_a = mirna_target_a.replace("U", "T") print "cDNA:" + mirna_target_a #reverse complement from string import maketrans f_strand = "TACG" r_strand = "ATGC" trans = maketrans(f_strand, r_strand) mirna_target_c = mirna_target_a.translate(trans) print "Complement:" + mirna_target_c mirna_target_rc = mirna_target_c[::-1] print "Reverse Complement:" + mirna_target_rc #generate RT primers rt_primers = ["CTTTT" + str(x) for x in mirna_target] for i in range(15): rt_primers_dict = {primer:(("t" * i )+ str(primer)) for primer in rt_primers} print rt_primers_dict
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